Efficient mAb production in CHO cells with optimized signal peptide, codon, and UTR
نویسندگان
چکیده
منابع مشابه
A Novel mAb against a Human CD34 Peptide Reacts with the Native Protein on CD34+ Cells
Background: Human CD34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (HSCs) and the small- vessel endothelial cells of a variety of tissues. CD34 plays a critical role as a marker for diagnosis and classification of leukemia. Anti CD34 antibodies are used for isolation and purification of HSCs from bone marrow, peripheral blood and cord blood. Objective: ...
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Monoclonal antibodies are the most demanding biotherapeutic drugs now a days used for the cure of various critical illnesses. Chinese hamster ovary (CHO) cells are one of the main hosts used for the large scale production of these antibodies. However, the cell line and production processes are the key factors to determine the cost and affordability of these antibodies. The metabolic waste lacti...
متن کاملa novel mab against a human cd34 peptide reacts with the native protein on cd34+ cells
background: human cd34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (hscs) and the small- vessel endothelial cells of a variety of tissues. cd34 plays a critical role as a marker for diagnosis and classification of leukemia. anti cd34 antibodies are used for isolation and purification of hscs from bone marrow, peripheral blood and cord blood. objective: t...
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Introduction Mammalian cell culture medium development has widely evolved in recent years. The use of hydrolysates as serum replacement has led to process variability due to lot-tolot variations. The undefined composition of these media could also increase the process optimization timelines, sometimes with limited impact on process performances. With the reduction of process development activit...
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Introduction The development of chemically well defined media is a demanding task in order to create the optimal conditions for an in vitro stem cell (SC) proliferation and differentiation system. Signals that govern SC differentiation into multiple mature cell types are provided by growth factors. TGFb-1 regulates a number of biological processes, including cell differentiation and proliferati...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2018
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-018-8986-5